Sample collection and storages Serum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cycles Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles. Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles鸡总蛋白酶(Protease)ELISA试剂盒检测前准备工作：
1. 请提前20分钟从冰箱中取出试剂盒，平衡至室温。
2. 从冰箱中取出的浓缩洗涤液可能有结晶，属于正常现象；放置室温，轻摇均匀，待结晶完全溶解后再配置洗涤液。可将20ml浓缩洗涤液用蒸馏水或去离子水稀释配置成400ml工作浓度的洗涤液，未用完的放回4℃
3. 20×洗涤缓冲液的稀释：蒸馏水按1：20稀释，即1份20×洗涤缓冲液加19份蒸馏水。
上海轩泽康生物拥有专业的研发团队和实验室，根据我们专业的技术自主研发ELISA检测试剂盒，鸡总蛋白酶(Protease)ELISA试剂盒经过不断的实验优化和改进，积累了大量的经验，能对血清及其他样本定量检测抗原，定性检测特异性抗体。ELISA检测试剂盒的方便性、稳定性以及重复性都具有很大的优势，是科研小伙伴们的喜好选择。